《植物生理学报》 2015, 51(3): 385-390

5-氨基乙酰丙酸(ALA)对盐胁迫下葡萄叶片中AsA-GSH循环的影响

赵宝龙, 刘鹏, 王文静, 孙军利*, 马海新

石河子大学农学院, 新疆石河子832003

通信作者：孙军利；E-mail: junli7656@126.com；Tel: 0993-2057992

摘　要：

以两个耐盐程度不同的葡萄品种‘夏黑’ (耐盐性较弱)和‘里扎马特’ (耐盐性较强)为材料, 分析了不同浓度ALA对不同浓度盐胁迫下葡萄叶片中AsA-GSH循环的影响。研究表明: 喷施75 mg•L^-1 ALA可显著提高2 g•kg^-1盐胁迫下‘夏黑’葡萄叶片中抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)、单脱氢抗坏血酸还原酶(MDHAR)、脱氢抗坏血酸还原酶(DHAR)的活性以及还原型抗坏血酸(AsA)、还原型谷胱甘肽(GSH)含量和AsA/DHA、GSH/GSSG比值, 显著降低氢抗坏血酸(DHA)与氧化型谷胱甘肽(GSSG)含量; 而喷施150 mg•L^-1 ALA可显著提高4 g•kg^-1盐胁迫下‘里扎马特’葡萄叶片中APX、GR、MDHAR、DHAR的活性以及AsA、GSH含量和AsA/DHA、GSH/GSSG比值, 显著降低DHA与GSSG含量。

关键词：5-氨基乙酰丙酸; 盐胁迫; 葡萄; AsA-GSH循环

收稿：2015-02-03   修定：2015-03-02

资助：国家自然科学基金(31460495)和石河子大学优秀青年基金(2012ZRKXYQ09)。

Effects of 5-Aminolevulinic Acid on the AsA-GSH Cycle in Grape Leaves under Salt Stress

ZHAO Bao-Long, LIU Peng, WANG Wen-Jing, SUN Jun-Li*, MA Hai-Xin

College of Agriculture, Shihezi University, Shihezi, Xinjiang 832003, China

Corresponding author: SUN Jun-Li; E-mail: junli7656@126.com; Tel: 0993-2057992

Abstract:

Using two salinity-resistance grape cultivars: ‘Summer Black’ (weak salt resistance) and ‘Rizamat’ (strong salt resistance) as the material, effects of the different concentrations of ALA on AsA-GSH cycle under different concentrations of salt stress in grape leaves were analyzed. The results showed that spraying 75 mg•L^-1 ALA can significantly improve activities of ascorbic acid peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), dehydroascorbic acid reductas (DHAR) and contents of ascorbic acid (AsA) and glutathione (GSH) and AsA/DHA, GSH/GSSG ratio, significantly reduce contents of hydrogen ascorbic acid type (DHA) and oxidized glutathione (GSSG) in ‘Summer Black’ grape leaves under 2 g•kg^-1 salt stress. And spraying 150 mg•L^-1 ALA can significantly improve activities of APX, GR, MDHAR, DHAR and contents of AsA and GSH and AsA/DHA, GSH/GSSG ratio, significantly reduce contents of DHA and GSSG in ‘Rizamat’ grape leaves under 4 g•kg^-1 salt stress.

Key words: 5-aminolevulinic acid (ALA); salt stress; grape; AsA-GSH cycle